Insulin Receptor beta [p Thr1160] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60777

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Porcine

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Primate (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Insulin Receptor beta Antibodies »

Product Summary for Insulin Receptor beta [p Thr1160] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr1160 conjugated to KLH. Accession # P06213

Modification

p Thr1160

Specificity

Specific for endogenous levels of the ~95 kDa Insulin receptor phosphorylated at Thr1160. Immunolabeling is completely eliminated with lambda-phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Insulin Receptor beta [p Thr1160] Antibody

Western Blot: Insulin Receptor beta [p Thr1160] Antibody [NBP2-60777]

Western Blot: Insulin Receptor beta [p Thr1160] Antibody [NBP2-60777]

Western Blot: Insulin Receptor Beta [p Thr1160] Antibody [NBP2-60777] - Western blot of HeLa cell lysate showing specific labeling of the~95kDa IR protein phosphorylated at Thr1160 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).

Applications for Insulin Receptor beta [p Thr1160] Antibody

Application
Recommended Usage

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Insulin Receptor beta

The Insulin Receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. InsR is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residues 960, 972, 1316, and 1322 regulate the assembly of signal transduction complexes.

Alternate Names

CD220, CD220 antigen, EC 2.7.10, EC 2.7.10.1, HHF5, insulin receptor, IR

Gene Symbol

INSR

Additional Insulin Receptor beta Products

Product Documents for Insulin Receptor beta [p Thr1160] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Insulin Receptor beta [p Thr1160] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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