Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all INT4 Antibodies »

Product Summary for INT4 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 913 and 963 of integrator complex subunit 4 using the numbering given in entry NP_291025.3 (GeneID 92105).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

108 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for INT4 Antibody

Western Blot: INT4 Antibody [NB100-60660]

Western Blot: INT4 Antibody [NB100-60660]

Western Blot: INT4 Antibody [NB100-60660] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-60660 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
Immunohistochemistry-Paraffin: INT4 Antibody [NB100-60660]

Immunohistochemistry-Paraffin: INT4 Antibody [NB100-60660]

Immunohistochemistry-Paraffin: INT4 Antibody [NB100-60660] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Immunohistochemistry-Paraffin: INT4 Antibody [NB100-60660]

Immunohistochemistry-Paraffin: INT4 Antibody [NB100-60660]

Immunohistochemistry-Paraffin: INT4 Antibody [NB100-60660] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-INT4 used at a dilution of 1:250.

Applications for INT4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:250

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: INT4

INT4 has been identified as a component of the twelve-subunit integrator complex that functions in RNA Pol II mediated 3'-end mRNA processing of the U1 and U2 small nuclear RNAs (snRNAs).

Alternate Names

INT4, integrator complex subunit 4, MGC16733, MST093, MSTP093

Entrez Gene IDs

92105 (Human); 101861 (Mouse)

Gene Symbol

INTS4

UniProt

Q96HW7 (Human)

Additional INT4 Products

Product Documents for INT4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for INT4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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