Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunoprecipitation

Label

PE/Atto594 (Excitation = 488 nm, Emission = 627 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 31H4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Integrin alpha 2/CD49b Antibodies »

Product Summary for Integrin alpha 2/CD49b Antibody (31H4) [PE/Atto594]

Immunogen

Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.

Specificity

It is specific to the alpha 2 subunit by relative expression of antigen on various cell lines by Flow Cytomery, and its recognition of affinity purified alpha 2 beta 1 in Dot Blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Integrin alpha 2/CD49b Antibody (31H4) [PE/Atto594]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Integrin alpha 2/CD49b

Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.

Alternate Names

CD49b, ITGA2, VLA-2 alpha

Gene Symbol

ITGA2

Additional Integrin alpha 2/CD49b Products

Product Documents for Integrin alpha 2/CD49b Antibody (31H4) [PE/Atto594]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Integrin alpha 2/CD49b Antibody (31H4) [PE/Atto594]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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