Integrin alpha 4/CD49d Antibody (5C4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37503

Novus Biologicals, part of Bio-Techne
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NBP2-37503
NBP2-37503-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5C4

Format

BSA Free

Concentration

1 mg/ml

View all Integrin alpha 4/CD49d Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Integrin alpha 4/CD49d expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin alpha 4/CD49d Antibody (5C4) - BSA Free

Western Blot: Integrin alpha 4/CD49d Antibody (5C4)BSA Free [NBP2-37503]

Western Blot: Integrin alpha 4/CD49d Antibody (5C4)BSA Free [NBP2-37503]

Western Blot: Integrin alpha 4/CD49d Antibody (5C4) [NBP2-37503] - Analysis using ITGA4 mouse mAb against K562(1) and Jurkat (2) cell lysate.
Flow Cytometry: Integrin alpha 4/CD49d Antibody (5C4) - BSA Free [NBP2-37503]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (5C4) - BSA Free [NBP2-37503]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (5C4) [NBP2-37503] - Analysis of Jurkat cells using ITGA4 mouse mAb (green) and negative control (red).
Western Blot: Integrin alpha 4/CD49d Antibody (5C4)BSA Free [NBP2-37503]

Western Blot: Integrin alpha 4/CD49d Antibody (5C4)BSA Free [NBP2-37503]

Western Blot: Integrin alpha 4/CD49d Antibody (5C4) [NBP2-37503] - Analysis using ITGA4 mAb against human ITGA4 (AA: 926-978) recombinant protein. (Expected MW is 31.7 kDa)
View 1 more images

Applications for Integrin alpha 4/CD49d Antibody (5C4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 4/CD49d

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Alternate Names

CD49d, ITGA4, VLA-4

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (5C4) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin alpha 4/CD49d Antibody (5C4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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