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Integrin alpha 4/CD49d Antibody (9F10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43398

Clone 9F10 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-43398-0.1mg

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 9F10

Format

BSA Free

Concentration

0.5 mg/ml

Product Specifications

Immunogen

The immunogen for this antibody was CD49d.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Integrin alpha 4/CD49d Antibody (9F10) - BSA Free

Flow Cytometry: Integrin alpha 4/CD49d Antibody (9F10) - BSA Free [NBP1-43398]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (9F10) - BSA Free [NBP1-43398]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (9F10) [NBP1-43398] - Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.125 ug of Anti-Human CD49d Purified (filled histogram) followed by Anti-Mouse IgG FITC. Cells in the lymphocyte gate were used for analysis.
Immunofluorescence: Integrin alpha 4/CD49d Antibody (9F10) - BSA Free [NBP1-43398]

Immunofluorescence: Integrin alpha 4/CD49d Antibody (9F10) - BSA Free [NBP1-43398]

Immunofluorescence: Integrin alpha 4/CD49d Antibody (9F10) [NBP1-43398] - Analysis was performed using 70% confluent log phase MOLT-4 cells.

Applications for Integrin alpha 4/CD49d Antibody (9F10) - BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The 9F10 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 ug per test.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha 4/CD49d

The 9F10 monoclonal antibody reacts with human CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin beta7 and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).

Alternate Names

CD49d, ITGA4, VLA-4

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (9F10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin alpha 4/CD49d Antibody (9F10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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