Integrin alpha 4/CD49d Antibody (FW3-218)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60989

Novus Biologicals, part of Bio-Techne
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NBP2-60989
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Key Product Details

Species Reactivity

Validated:

Bovine, Goat, Sheep, Water Buffalo

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # FW3-218

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Integrin alpha 4/CD49d Antibodies »

Product Summary for Integrin alpha 4/CD49d Antibody (FW3-218)

Immunogen

Sheep mononuclear leukocyytes

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This monoclonal antibody is made in ascites form using mice. The ascites is collected and centrifuged to remove cells and large particles. The ascitic fluid is purified further by filtration through sequential filters with the last filter at 2 um. The concentration of the monoclonal antibody present in the ascites is determined with commercial radial immunodiffusion kits (1mg/ml).

Scientific Data Images for Integrin alpha 4/CD49d Antibody (FW3-218)

Flow Cytometry: Integrin alpha 4/CD49d Antibody (FW3-218) [NBP2-60989]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (FW3-218) [NBP2-60989]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (FW3-218) [NBP2-60989] - FC profile obtained with bovine leukocytes.

Applications for Integrin alpha 4/CD49d Antibody (FW3-218)

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 4/CD49d

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Alternate Names

CD49d, ITGA4, VLA-4

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (FW3-218)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin alpha 4/CD49d Antibody (FW3-218)

This monoclonal antibody is made in ascites form using mice. The ascites is collected and centrifuged to remove cells and large particles. The ascitic fluid is purified further by filtration through sequential filters with the last filter at 2 um. The concentration of the monoclonal antibody present in the ascites is determined with commercial radial immunodiffusion kits (1mg/ml).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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