Flow Cytometry: Integrin alpha 4/CD49d Antibody (Natalizumab) [NBP2-75916] - Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Natalizumab (panel B). After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications for Integrin alpha 4/CD49d Antibody (Natalizumab) - Azide and BSA Free

Application

Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Integrin alpha 4/CD49d

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Alternate Names

CD49d, ITGA4, VLA-4, biosimilar antibody, biosimilars

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (Natalizumab) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Integrin alpha 4/CD49d Antibody (Natalizumab) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Integrin alpha 4/CD49d Antibody (Natalizumab) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75916

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Format

Concentration

View all Integrin alpha 4/CD49d Antibodies »

Product Summary for Integrin alpha 4/CD49d Antibody (Natalizumab) - Azide and BSA Free

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Integrin alpha 4/CD49d Antibody (Natalizumab) - Azide and BSA Free

Flow Cytometry: Integrin alpha 4/CD49d Antibody (Natalizumab) - Azide and BSA Free [NBP2-75916]

Applications for Integrin alpha 4/CD49d Antibody (Natalizumab) - Azide and BSA Free

Block/Neutralize

Flow Cytometry

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Integrin alpha 4/CD49d

Alternate Names

Gene Symbol

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (Natalizumab) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

Product Specific Notices for Integrin alpha 4/CD49d Antibody (Natalizumab) - Azide and BSA Free