Flow Cytometry: Integrin alpha 4/CD49d Antibody (Natalizumab) - Chimeric [NBP2-75917] - Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of Natalizumab (NBP2-75917, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications

Application

Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original human IgG4 format, for improved compatibility with existing reagents, assays and techniques.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Integrin alpha 4/CD49d

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Alternate Names

CD49d, ITGA4, VLA-4, biosimilar antibody, biosimilars

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Integrin alpha 4/CD49d Antibody (Natalizumab) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75917

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Format

Concentration

View all Integrin alpha 4/CD49d Antibodies »

Product Summary

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images

Flow Cytometry: Integrin alpha 4/CD49d Antibody (Natalizumab) - Chimeric - Azide and BSA Free [NBP2-75917]

Applications

Block/Neutralize

Flow Cytometry

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Integrin alpha 4/CD49d

Alternate Names

Gene Symbol

Additional Integrin alpha 4/CD49d Products

Product Documents

Product Datasheets

COA

Certificate of Analysis

Product Specific Notices