Integrin alpha 4/CD49d Antibody (R1-2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09131

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-09131
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # R1-2

Format

BSA Free

Concentration

1 mg/ml

View all Integrin alpha 4/CD49d Antibodies »

Product Specifications

Immunogen

murine T lymphoma line TK1

Specificity

The rat monoclonal antibody R1-2 recognizes an extracellular epitope of murine CD49d / Integrin alpha 4 (VLA-4 alpha), an approximately 150 kDa glycoprotein of the integrin family, expressed on multiple blood cell types.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images for Integrin alpha 4/CD49d Antibody (R1-2) - BSA Free

Integrin alpha 4/CD49d Antibody (R1-2) - BSA Free

Flow Cytometry: Rat Monoclonal Integrin alpha 4/CD49d Antibody (R1-2) [NBP3-09131] -

Flow Cytometry: Rat Monoclonal Integrin alpha 4/CD49d Antibody (R1-2) [NBP3-09131] - Flow cytometry surface staining pattern of murine peripheral blood cells stained using anti-mouse CD49d (R1-2) purified antibody (concentration in sample 5 ug/ml, DAR APC).
Integrin alpha 4/CD49d Antibody (R1-2) - BSA Free

Flow Cytometry - Rat Monoclonal Integrin alpha 4/CD49d Antibody (R1-2) [NBP3-09131] -

Flow Cytometry - Rat Monoclonal Integrin alpha 4/CD49d Antibody (R1-2) [NBP3-09131] - Separation of cells stained using anti-mouse CD49d (R1-2) purified antibody (concentration in sample 5 μg/ml, DAR APC, red-filled) from cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of murine peripheral blood cells.

Applications for Integrin alpha 4/CD49d Antibody (R1-2) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 4/CD49d

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Alternate Names

CD49d, ITGA4, VLA-4

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (R1-2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Integrin alpha 4/CD49d Antibody (R1-2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.