Integrin alpha 5 beta 1 Antibody (BMC5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29788

Novus Biologicals, part of Bio-Techne
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NBP2-29788-0.025ml
NBP2-29788
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin (Negative), Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # BMC5

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Integrin alpha 5 beta 1 Antibodies »

Product Specifications

Immunogen

Mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.

Specificity

Antibody was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Description

Relevant Entrez Gene Numbers: NM_002205.2

Scientific Data Images for Integrin alpha 5 beta 1 Antibody (BMC5)

Immunohistochemistry: Integrin alpha 5 beta 1 Antibody (BMC5) [NBP2-29788]

Immunohistochemistry: Integrin alpha 5 beta 1 Antibody (BMC5) [NBP2-29788]

Immunohistochemistry: Integrin alpha 5 beta 1 Antibody (BMC5) [NBP2-29788] - Rat anti-Mouse Integrin alpha5beta1 staining of mouse gut.

Applications for Integrin alpha 5 beta 1 Antibody (BMC5)

Application
Recommended Usage

Flow Cytometry

10ul/ml

Functional

10ul/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

2 uL
Application Notes
Inhibition of cell adhesion to fibronectin: 10 ul/mL Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete. Immunohistochemistry on acetone-fixed frozen sections only; formalin fixation is not recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Integrin alpha 5 beta 1

Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.

Alternate Names

VLA-5

Gene Symbol

ITGA5

UniProt

P08648

Additional Integrin alpha 5 beta 1 Products

Product Documents for Integrin alpha 5 beta 1 Antibody (BMC5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin alpha 5 beta 1 Antibody (BMC5)

Relevant Entrez Gene Numbers: NM_002205.2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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