Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75909

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Product Datasheet / COA

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Human IgG₄ kappa Clone # M200 (Volociximab)

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Integrin alpha 5 beta 1 Antibodies »

Product Summary

Immunogen

Heterodimeric (alpha)5(beta)1Fc fusion protein

Specificity

The antibody binds to human (alpha)5(beta)1 integrin with a Kd of 0.367 nM (determined by Biacore), an EC50 of 0.2 nM and an IC50 of 2.3 nM.

Clonality

Monoclonal

Host

Human

Isotype

IgG₄ kappa

Scientific Data Images

Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Azide and BSA Free [NBP2-75909]

Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) [NBP2-75909] - PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (blue line). After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody and cells analyzed on a FACSCanto flow-cytometer.

Immunocytochemistry/Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) [NBP2-75909] -

Immunocytochemistry/Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) [NBP2-75909] - Staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab).

Applications

Application

Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Integrin alpha 5 beta 1

Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.

Alternate Names

VLA-5, biosimilar antibody, biosimilars

Gene Symbol

ITGA5

Additional Integrin alpha 5 beta 1 Products

Product Documents

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COA

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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