Integrin alpha 7 Antibody (8G2)

Novus Biologicals, part of Bio-Techne | Catalog # H00003679-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 8G2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Integrin alpha 7 Antibodies »

Product Specifications

Immunogen

ITGA7 (NP_002197, 478 a.a. ~ 577 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SHEVSIAPRSIDLEQPNCAGGHSVCVDLRVCFSYIAVPSSYSPTVALDYVLDADTDRRLRGQVPRVTFLSRNLEEPKHQASGTVWLKHQHDRVCGDAMFQ

Specificity

ITGA7 - integrin, alpha 7

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Integrin alpha 7 Antibody (8G2)

Western Blot: Integrin alpha 7 Antibody (8G2) [H00003679-M01]

Western Blot: Integrin alpha 7 Antibody (8G2) [H00003679-M01]

Western Blot: Integrin alpha 7 Antibody (8G2) [H00003679-M01] - ITGA7 monoclonal antibody (M01), clone 8G2 Analysis of ITGA7 expression in Jurkat.
Sandwich ELISA: Integrin alpha 7 Antibody (8G2) [H00003679-M01] - Detection limit for recombinant GST tagged ITGA7 is approximately 0.1ng/ml as a capture antibody.

Applications for Integrin alpha 7 Antibody (8G2)

Application
Recommended Usage

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Integrin alpha 7

ITGA7 encodes integrin alpha chain 7. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 7 undergoes post-translational cleavage within the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that binds to the extracellular matrix protein laminin-1. Alpha 7 beta 1 is the major integrin complex expressed in differentiated muscle cells. Splice variants of alpha 7 that differ in both the extracellular and cytoplasmic domains exist in the mouse; however, to date only a single human transcript type has been isolated: it contains extracellular and cytoplasmic domains corresponding to the mouse X2 and B variants, respectively. A unique extracellular splice variant has been identified in human, although it clearly represents a minor species and its biological significance is unclear.

Alternate Names

ITGA7

Entrez Gene IDs

3679 (Human)

Gene Symbol

ITGA7

OMIM

600536 (Human)

UniProt

NP_002197 (Human)

Additional Integrin alpha 7 Products

Product Documents for Integrin alpha 7 Antibody (8G2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin alpha 7 Antibody (8G2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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