Integrin alpha L/CD11a Antibody (2B2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26189
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2B2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Integrin alpha L/CD11a Antibody (2B2)
Immunogen
A synthesized peptide derived from Human Integrin alpha L/CD11a [UniProt P20701]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Integrin alpha L/CD11a Antibody (2B2)
Western Blot: Integrin alpha L/CD11a Antibody (2B2) [NBP3-26189] -
Western Blot: Integrin alpha L/CD11a Antibody (2B2) [NBP3-26189] - Positive Western Blot detected in: THP-1 whole cell lysate.All lanes: Integrin alpha L/CD11a Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 129, 135, 120 kDa
Observed band size: 130 kDa
Immunocytochemistry/Immunofluorescence: Integrin alpha L/CD11a Antibody (2B2) [NBP3-26189] -
Immunocytochemistry/Immunofluorescence: Integrin alpha L/CD11a Antibody (2B2) [NBP3-26189] - Staining of THP-1 cell with Integrin alpha L/CD11a Antibody (2B2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 490-conjugated Goat Anti-Rabbit IgG (H+L).Applications for Integrin alpha L/CD11a Antibody (2B2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Integrin alpha L/CD11a
Alternate Names
CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21
Gene Symbol
ITGAL
Additional Integrin alpha L/CD11a Products
Product Documents for Integrin alpha L/CD11a Antibody (2B2)
Product Specific Notices for Integrin alpha L/CD11a Antibody (2B2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...