Skip to main content

Integrin alpha L/CD11a Antibody (MHM24) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09043

Recombinant Monoclonal Antibody. Clone MHM24 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-09043-0.025mg
NBP3-09043-0.2mg

Key Product Details

Species Reactivity

Human

Applications

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # MHM24

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Spleen cells from BALB/c mice immunised with EBV-transformed human lymphoblastoid cells were fused with P3-NSUlAg4.1 myeloma cells and the resulting hybridomas were screened for antibody production by a radioimmunoassay on T and B cell lines.

Specificity

MHM24 binds to the CD11a subunit of lymphocyte function-associated antigen. It binds to both the high- and low-affinity forms of the antigen.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images

Immunocytochemistry/ Immunofluorescence: Integrin alpha L/CD11a Antibody (MHM24) - Chimeric - Azide and BSA Free [NBP3-09043]

Immunocytochemistry/ Immunofluorescence: Integrin alpha L/CD11a Antibody (MHM24) - Chimeric - Azide and BSA Free [NBP3-09043]

Immunocytochemistry/Immunofluorescence: Integrin alpha L/CD11a Antibody (MHM24) - Chimeric [NBP3-09043] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of MHM24 (NBP3-09043) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing diffuse membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09043, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.
Flow Cytometry: Integrin alpha L/CD11a Antibody (MHM24) - Chimeric - Azide and BSA Free [NBP3-09043]

Flow Cytometry: Integrin alpha L/CD11a Antibody (MHM24) - Chimeric - Azide and BSA Free [NBP3-09043]

Flow Cytometry: Integrin alpha L/CD11a Antibody (MHM24) - Chimeric [NBP3-09043] - Jurkat cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of MHM24 (NBP3-09043, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Cytofluorography was used to show the binding of MHM24 to peripheral blood lymphocytes, with 97.3% of cells stained by MHM24 (Hildreth et al, 1983). In inhibition experiments, MHM24 inhibited T cell-mediated lysis of virus-infected cells (Hildreth et al, 1983). Additionally, MHM24 inhibited NK cell-mediated lysis (Hildreth et al, 1983).Flow cytometry showed MHM24 exhibits non-selective binding to high-affinity and low-affinity forms of LFA-1 (Huang et al, 2006). Murine MHM24 was humanised and shown to prevent adhesion of human T cells to human keratinocytes and the proliferation of T cells in response to nonautologous leukocytes (Werther et al, 1996).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha L/CD11a

CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. CD11a is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.

Alternate Names

CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21

Gene Symbol

ITGAL

Additional Integrin alpha L/CD11a Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...