Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PE/Cy7 (Excitation = 488 nm, Emission = 778 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2549B

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Integrin alpha V beta 3 Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line CHO-derived human Integrin alpha V beta 3.
Human Integrin alpha V (Phe31-Val992) and Human Integrin beta 3 (Gly27-Asp718)
Accession # NP_002201 and AAA52589

Specificity

In ELISAs, it detects recombinant human Integrin  alphaV beta3 heterodimer, but does not detect recombinant human Integrin  alphaV beta1 and Integrin  alpha6 beta1 heterodimers or recombinant human Integrin  alphaV monomer.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Integrin alpha V beta 3 Antibody (2549B) [PE/Cy7]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Integrin alpha V beta 3

Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha V beta 3 is the predominant integrin of osteoclasts (bone resorbing cells) and is upregulated in malignant melanoma. Integrin alpha V beta 3 binds to a range of ECM proteins (e.g. vitronectin, fibrinogen, fibronectin) via an Arg-Gly-Asp (RGD) motif.

Alternate Names

antigen identified by monoclonal L230, CD51, CD51 antigen, integrin alpha-V, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), MSK8, Vitronectin receptor subunit alpha, VNRADKFZp686A08142

Additional Integrin alpha V beta 3 Products

Product Documents for Integrin alpha V beta 3 Antibody (2549B) [PE/Cy7]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Integrin alpha V beta 3 Antibody (2549B) [PE/Cy7]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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