Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin (Negative), Immunoprecipitation

Cited:

Functional (Inhibition)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 27.1 (VNR-1)

Format

BSA Free

Concentration

1 mg/ml

View all Integrin alpha V beta 3 Antibodies »

Product Specifications

Immunogen

This antibody is specific to Integrin Alpha VBeta 3

Specificity

Reacts with the human integrin alphaVbeta3. Shown to be specific for alphaVbeta3 by Loftus JC et al (1990) Science 249:915-918.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Relevant Entrez Gene Numbers: NM_002210.2

Applications for Integrin alpha V beta 3 Antibody (27.1 (VNR-1)) - BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

1:10-1:1000

Immunoprecipitation

1:10-1:500
Application Notes
Immunohistochemistry: 4-10% formalin fixed frozen tissues (short fixation times, 10 minutes room temperature) only; not effective on paraffin embedded tissues Immunoprecipitation Functional blocking: Competes with clone LM609 for integrin function blocking. Optimal working dilutions must be determined by end user.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

20mM Phosphate (pH 7.6) and 0.25M NaCl

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Integrin alpha V beta 3

Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha V beta 3 is the predominant integrin of osteoclasts (bone resorbing cells) and is upregulated in malignant melanoma. Integrin alpha V beta 3 binds to a range of ECM proteins (e.g. vitronectin, fibrinogen, fibronectin) via an Arg-Gly-Asp (RGD) motif.

Alternate Names

antigen identified by monoclonal L230, CD51, CD51 antigen, integrin alpha-V, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), MSK8, Vitronectin receptor subunit alpha, VNRADKFZp686A08142

Gene Symbol

ITGAV

UniProt

P06756

Additional Integrin alpha V beta 3 Products

Product Documents for Integrin alpha V beta 3 Antibody (27.1 (VNR-1)) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin alpha V beta 3 Antibody (27.1 (VNR-1)) - BSA Free

Relevant Entrez Gene Numbers: NM_002210.2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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