Integrin alpha V beta 3 Antibody (BV3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11213

Submit a review

Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Rat, Chicken

Applications

Flow Cytometry, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # BV3

Concentration

0.1 mg/ml

View all Integrin alpha V beta 3 Antibodies »

Product Specifications

Immunogen

The monoclonal antibody BV3 recognizes human alpha-V/beta-3 integrin present on human cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Integrin alpha V beta 3 Antibody (BV3)

Flow Cytometry: Integrin alpha V beta 3 Antibody (BV3) [NBP3-11213]

Flow Cytometry: Integrin alpha V beta 3 Antibody (BV3) [NBP3-11213] - HUVEC cells were incubated with 2ug/ml NBP3-11213 for 1h at 4C. Black: isotype control mouse IgG1, Red: cells only, Blue: NBP3-11213.

Applications for Integrin alpha V beta 3 Antibody (BV3)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

For Flow Cytometry and immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Integrin alpha V beta 3

The monoclonal antibody BV3 recognizes human alpha-V/beta-3 integrin present on human cells. Integrins are a superfamily of alphabeta heterodimeric cell-surface adhesion receptors found in many species. They are expressed on a variety of cells and mediate numerous physiological processes, including inflammation, migration, adhesion and proliferation. The beta3 family consist of 2 members: alphaIIbbeta3 and alphavbeta3, which mediate cell-cell and cell-ECM interactions and are important for cellular migration, regulation of gene expression, cell survival, adhesion and differentiation. All processes which are involved in tissue development, angiogenesis and thrombosis. Each subunit consist of an extracellular domain, a single transmembrane segment and a cytoplasmic tail. They connect to the actin cytoskeleton via adaptor proteins that bind theircytoplasmic tails. Cell matrix adhesions also act as signaling units by their capacity to organize the actin cytoskeleton and to accumulate various signaling intermediates. Integrin alphavbeta3 was originally identified as the vitronectin receptor. Nevertheless, other ligands include fibrinogen, fibronectin, laminin, thrombospondin, Von Willebrand factor, tenascin, osteopontin and several forms of collagen. The interactions of integrin alphavbeta3 to those ligands is mediated by the RGD (Arg-Gly-Asp) sequence motif present in these proteins. Deregulation of beta3 integrins is involved in e.g. autoimmune diseases, cardiovascular disorders, transplant rejection and tumorigenesis. In contribution to the latter, integrin alphavbeta3 contribute by supporting growth of small (tumor) blood vessels thereby potentiating the metastatic potential. Overexpression of integrin alphavbeta3 has been demonstrated in various tumors and activated endothelium.

Alternate Names

antigen identified by monoclonal L230, CD51, CD51 antigen, integrin alpha-V, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), MSK8, Vitronectin receptor subunit alpha, VNRADKFZp686A08142

Gene Symbol

ITGAV

Additional Integrin alpha V beta 3 Products

Product Documents for Integrin alpha V beta 3 Antibody (BV3)

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Integrin alpha V beta 3 Antibody (BV3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews