Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # P2W7

Format

BSA Free

Concentration

1.0 mg/ml

View all Integrin alpha V/CD51 Antibodies »

Product Specifications

Immunogen

An ocular melanoma cell line (V+B2) expressing high levels of human integrin alpha V beta 2.

Reactivity Notes

Not yet tested in other species.

Localization

Type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin alpha V/CD51 Antibody (P2W7) - BSA Free

Immunocytochemistry/ Immunofluorescence: Integrin alpha V/CD51 Antibody (P2W7) - BSA Free [NB100-2618]

Immunocytochemistry/ Immunofluorescence: Integrin alpha V/CD51 Antibody (P2W7) - BSA Free [NB100-2618]

Immunocytochemistry/Immunofluorescence: Integrin alpha V/CD51 Antibody (P2W7) [NB100-2618] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Integrin alpha V/CD51 Antibody [P2W7] (NB100-2618) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for Integrin alpha V/CD51 Antibody (P2W7) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.25 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

8-25 ug/ml

Immunohistochemistry

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Human alpha v is about 150-160kDa under non-reducing conditions. Under reducing conditions it is cleaved into 2 bands of about 125-130kDa +20-25kDa. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha V/CD51

Integrin alpha V chain interacts with the integrin beta 3 subunit/CD61 to form the alpha-V-beta-3 heterodimer/vitronectin receptor. It is expressed on endothelial cells, some activated leukocytes, NK cells, macrophages, neutrophils, and platelets. Integrin alpha V also forms heterodimers with the integrin beta 1, beta 5, beta 6, and beta 8 subunits. Alpha-V-beta-3 is an activation dependent receptor for platelet attachment and spreading on vitronectin and other matrix components.

Alternate Names

CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

Additional Integrin alpha V/CD51 Products

Product Documents for Integrin alpha V/CD51 Antibody (P2W7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Integrin alpha V/CD51 Antibody (P2W7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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