Integrin alpha V/CD51 Antibody (SC56-07)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23955
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # SC56-07
Concentration
1 mg/ml
Product Summary for Integrin alpha V/CD51 Antibody (SC56-07)
Immunogen
Synthetic peptide within of Human Integrin alpha V/CD51 aa 1000-1048 / 1048. (SwissProt: P06756 Human; SwissProt: P43406 Mouse)
Localization
Membrane.Membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Integrin alpha V/CD51 Antibody (SC56-07)
Western Blot: Integrin alpha V/CD51 Antibody (SC56-07) [NBP3-23955] -
Western blot analysis of Integrin alpha V on A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours.
Immunocytochemistry/Immunofluorescence: Integrin alpha V/CD51 Antibody (SC56-07) [NBP3-23955] -
ICC staining of Integrin alpha V in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.
Immunocytochemistry/Immunofluorescence: Integrin alpha V/CD51 Antibody (SC56-07) [NBP3-23955] -
ICC staining of Integrin alpha V in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.Applications for Integrin alpha V/CD51 Antibody (SC56-07)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Integrin alpha V/CD51
Alternate Names
CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA
Gene Symbol
ITGAV
Additional Integrin alpha V/CD51 Products
Product Documents for Integrin alpha V/CD51 Antibody (SC56-07)
Product Specific Notices for Integrin alpha V/CD51 Antibody (SC56-07)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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