Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Janelia Fluor 669

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8E3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Integrin beta 1/CD29 Antibodies »

Product Specifications

Immunogen

Purified Integrin beta 1/CD29 from HT-1080 cells.

Specificity

Under native conditions, clone 8E3 is shown to recognize the unbent, monomeric ER form of Integrin beta 1/CD29 at the PSI domain; however, the epitope only becomes exposed in the Golgi form after denaturation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Integrin beta 1/CD29 Antibody (8E3) [Janelia Fluor® 669]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Integrin beta 1/CD29

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (8E3) [Janelia Fluor® 669]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Integrin beta 1/CD29 Antibody (8E3) [Janelia Fluor® 669]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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