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Integrin beta 1/CD29 Antibody (CL15622) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44158

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL15622

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P05556, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Integrin beta 1/CD29 Antibody (CL15622) - Azide and BSA Free

Integrin beta 1/CD29 Antibody (CL15622) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP3-44158]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP3-44158]

Analysis in human placenta and cerebral cortex tissues using NBP3-44158 antibody. Corresponding Integrin beta 1/CD29 RNA-seq data are presented for the same tissues.
Integrin beta 1/CD29 Antibody (CL15622) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP3-44158]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP3-44158]

Staining of human cerebral cortex shows strong positivity in endothelial cells.
Integrin beta 1/CD29 Antibody (CL15622) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP3-44158]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP3-44158]

Staining of human liver shows strong positivity in sinusoids, as well as weaker membranous positivity in hepatocytes.

Applications for Integrin beta 1/CD29 Antibody (CL15622) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2500-1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 1/CD29

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (CL15622) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin beta 1/CD29 Antibody (CL15622) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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