Integrin beta 1/CD29 Antibody (K20) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52665

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-52665-0.025mg
NBP2-52665-0.2mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # K20

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Integrin beta 1/CD29 Antibodies »

Product Specifications

Immunogen

Human T-cell lymphoma cells injected into Biozzi high-responder mice.

Specificity

Recognises human CD29.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Integrin beta 1/CD29 Antibody (K20) - Chimeric - Azide and BSA Free

Flow Cytometry: Integrin beta 1/CD29 Antibody (K20) - Chimeric - Azide and BSA Free [NBP2-52665]

Flow Cytometry: Integrin beta 1/CD29 Antibody (K20) - Chimeric - Azide and BSA Free [NBP2-52665]

Flow Cytometry: Integrin beta 1/CD29 Antibody (K20) - Chimeric [NBP2-52665] - Flow-cytometry using anti-CD29 antibody K20. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of K20 (NBP2-52665, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications for Integrin beta 1/CD29 Antibody (K20) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry-Frozen

1:10 - 1:500
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Integrin beta 1/CD29

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (K20) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin beta 1/CD29 Antibody (K20) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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