Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB110-81701
Clone MEM-101A was used by HLDA to establish CD designation.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Porcine, Canine, Mouse (Negative)
Applications
Flow Cytometry, Immunoprecipitation, Western Blot
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-101A
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Raji Burkitt's lymphoma cell line (NM_002211.3).
Specificity
The antibody MEM-101A reacts with CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells). HLDA VI; WS Code AS A048
Marker
Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin]
Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin] [NB110-81701] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD29 (MEM-101A).Lane 1: lysate of human PBLLane 2: lysate of canine PBLLane 3: lysate of porcine PBL
Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin] [NB110-81701] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) biotin, streptavidin-APC.
Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin] [NB110-81701] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC.
Applications for Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin]
Application
Recommended Usage
Flow Cytometry
1-2 ug/ml
Immunoprecipitation
1:50
Western Blot
1:1000
Application Notes
Purity is > 95% (by SDS-PAGE).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Integrin beta 1/CD29
Alternate Names
CD29, ITGB1
Gene Symbol
ITGB1
Additional Integrin beta 1/CD29 Products
Product Documents for Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin]
Product Specific Notices for Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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