Integrin beta 2/CD18 Antibody (1B4) - Chimeric - Azide and BSA Free

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

The main application 1B4 has been proposed for is its anti-inflammatory activity. It has been shown to work as a Fab fragment prepared by papain digestion (Wright et al, 1983). Its binding to antigen was confirmed by RIA (Van Voorhis et al, 1983). 1B4 has been shown to work in Rhesus monkeys, and has reduced immunogenicity once humanized. 1B4 is also compatible with immunofluorescence which has been used to demonstrate that the fine specificity of the combining sites was not altered by humanization (Singer et al, 1993). It has been shown to prevent polymorphonuclear (PMN)-dpepndent pathology in animal models of hemorrhagic shock, dermal inflammation, reperfusion injury, IBD and meningitis (Vedder et al, 1988, Vedder et al, 1990, Mileski et al, 1990, Carlos et al, 1990, Tuomanen et al, 1989, Arfors et al, 1987, Wallace et al, 1991, Wallace et al, 1992). The glycosylation patterns of 1B4 have also been studied in detail using NMR, ES-MS, HPAEC-PAD mapping and glycosyl composition analysis (Yu Ip et al, 1994).