Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12031
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free [NBP3-12031]](https://resources.bio-techne.com/images/products/Integrin-beta-2-CD18-Antibody-60-3-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12031-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free [NBP3-12031]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Block/Neutralize, ELISA, Functional (Inhibition), Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 60.3
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free
Immunogen
The original antibody was generated by immunizing BALB/c mice with alloantigen activated T cells.
Specificity
This antibody binds the Integrin beta 2/CD18 component of the beta-2 integrin family which is expressed by leukocytes and neutrophils.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free [NBP3-12031]
Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (60.3) - Chimeric [NBP3-12031] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 60.3 (NBP3-12031) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12031, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Functional (Inhibition)
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Integrin beta 2/CD18
Alternate Names
CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta
Gene Symbol
ITGB2
Additional Integrin beta 2/CD18 Products
Product Documents for Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free
Product Specific Notices for Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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