Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # M18/2

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Integrin beta 2/CD18 Antibodies »

Product Specifications

Immunogen

Murine cytotoxic T cell glycoproteins

Specificity

The rat monoclonal antibody M18/2 recognizes CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein

Scientific Data Images

Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]

Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]

Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin [NBP1-41272] - Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2) FITC.
Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]

Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]

Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin [NBP1-41272] - Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2).
Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free Flow Cytometry: Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]

Flow Cytometry: Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]

Separation of murine myeloid cells stained using anti-mouse CD18 (M18/2) purified antibody (low endotoxin, concentration in sample 16 ug/ml, DAR APC, red-filled) from murine myeloid cells unstained by primary antibody (DAR APC, black-dashed) in Analysis (surface staining) of murine splenocyte suspension.

Applications

Application
Recommended Usage

Flow Cytometry

1 ug/ml

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 0.2 um filter sterilized

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin beta 2/CD18

CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Gene Symbol

ITGB2

Additional Integrin beta 2/CD18 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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