Integrin beta 2/CD18 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88127

Novus Biologicals, part of Bio-Techne
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NBP1-88127
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Integrin beta 2/CD18 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LEDNLYKRSNEFDYPSVGQLAHKLAENNIQPIFAVTSRMVKTYEKLTEIIPKSAVGELSEDSSNVVHLIKNAYNKLSSRVFLDHNALPDTLKVTYDSFCSNGV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin beta 2/CD18 Antibody

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Analysis in human lymph node and skeletal muscle tissues. Corresponding Integrin beta 2/CD18 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human gastrointestinal, lymph node, skeletal muscle and tonsil using Anti-Integrin beta 2/CD18 antibody NBP1-88127 (A) shows similar protein distribution across tissues to independent antibody NBP1-88128 (B).
Western Blot: Integrin beta 2/CD18 Antibody [NBP1-88127]

Western Blot: Integrin beta 2/CD18 Antibody [NBP1-88127]

Western Blot: Integrin beta 2/CD18 Antibody [NBP1-88127] - Analysis using Anti-ITGB2 antibody NBP1-88127 (A) shows similar pattern to independent antibody NBP1-88128 (B).
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Applications for Integrin beta 2/CD18 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 2/CD18

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Gene Symbol

ITGB2

Additional Integrin beta 2/CD18 Products

Product Documents for Integrin beta 2/CD18 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin beta 2/CD18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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