NB100-65599 recognizes the human beta4 integrin (also known as CD104), a 205kD glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumour cell lines. NB100-65599 recognizes an extracellular epitope on the CD104 molecule.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Integrin beta 4/CD104 Antibody (450-9D) [FITC]

Application

Recommended Usage

Flow Cytometry

1:10-1:1000

Application Notes

For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 4/CD104

Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.

Alternate Names

CD104, ITGB4

Gene Symbol

ITGB4

UniProt

P16144

Additional Integrin beta 4/CD104 Products

Product Documents for Integrin beta 4/CD104 Antibody (450-9D) [FITC]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Integrin beta 4/CD104 Antibody (450-9D) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Spectra Viewer
Flow Cytometry

Integrin beta 4/CD104 Antibody (450-9D) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-63326

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all Integrin beta 4/CD104 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Specificity

Clonality

Host

Isotype

Applications for Integrin beta 4/CD104 Antibody (450-9D) [FITC]

Flow Cytometry

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Integrin beta 4/CD104

Alternate Names

Gene Symbol

UniProt

Additional Integrin beta 4/CD104 Products

Product Documents for Integrin beta 4/CD104 Antibody (450-9D) [FITC]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Integrin beta 4/CD104 Antibody (450-9D) [FITC]

CONTACT INFORMATION

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