Integrin beta 5 Antibody (2C4)
Novus Biologicals, part of Bio-Techne | Catalog # H00003693-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2C4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ITGB5 (NP_002204, 421 a.a. ~ 516 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TASFEVSLEARSCPSRHTEHVFALRPVGFRDSLEVGVTYNCTCGCSVGLEPNSARCNGSGTYVCGLCECSPGYLGTRCECQDGENQSVYQNLCREA
Specificity
ITGB5 - integrin, beta 5
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Integrin beta 5 Antibody (2C4)
Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01]
Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01] - Analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01]
Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01] - Analysis of ITGB5 over-expressed 293 cell line, cotransfected with ITGB5 Validated Chimera RNAi ( Cat # H00003693-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ITGB5 monoclonal antibody (M01), clone 2C4 (Cat # H00003693-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01]
Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01] - ITGB5 monoclonal antibody (M01), clone 2C4 Analysis of ITGB5 expression in HeLa.Applications for Integrin beta 5 Antibody (2C4)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Integrin beta 5
Additional Integrin beta 5 Products
Product Documents for Integrin beta 5 Antibody (2C4)
Product Specific Notices for Integrin beta 5 Antibody (2C4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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