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Integrin beta 5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16976

Novus Biologicals, part of Bio-Techne
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NBP2-16976

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (98%), Mouse (98%), Porcine (98%), Rat (98%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Integrin beta 5. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (89%), Xenopus laevis (80%), Zebrafish (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin beta 5 Antibody

Integrin beta 5 Antibody

Western Blot: Integrin beta 5 Antibody [NBP2-16976] -

Western Blot: Integrin beta 5 Antibody [NBP2-16976] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 5 antibody [N1C1] (NBP2-16976) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Integrin beta 5 Antibody

Western Blot: Integrin beta 5 Antibody [NBP2-16976] -

Western Blot: Integrin beta 5 Antibody [NBP2-16976] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 5 antibody [N1C1] (NBP2-16976) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Integrin beta 5 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Integrin beta 5

Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.

Alternate Names

ITGB5

Gene Symbol

ITGB5

UniProt

Additional Integrin beta 5 Products

Product Documents for Integrin beta 5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin beta 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.