Integrin beta 7 Antibody (FIB27) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81087

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # FIB27

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Integrin beta 7 Antibodies »

Product Specifications

Immunogen

FIB27 was prepared by immunizing rats with TK1 cells.

Specificity

FIB27 binds specifically to both human and mouse Integrin beta 7 at epitope region Dl on which is involved in all a4b7-mediated adhesion events. Does not compete with DAKT32 for binding. FIB27 blocks LS722-induced TK1 cell aggregation (mAb LS722 is an activating anti-a4B7 antibody and induces aggregation via an a4B7-dependent pathway). Integrin beta 7 is one subunit is a heterodimer which makes up an intergin molecule. In the mouse beta-7 is selectively found on the majority of mature lymphocytes, whereas a small subpopulation of thymocytes and bone marrow cells express beta-7. Beta-7 is also expressed in variable amounts in CD4 T memory cells. Integrin molecules mediate cell-cell and cell-extracellular matrix adhesion and are involved in lymphocyte homing, leukocyte recruitment to inflammatory sites, myogenesis, hemopoiesis, and melanoma metastasis.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Integrin beta 7 Antibody (FIB27) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Integrin beta 7 Antibody (FIB27) - Chimeric - Azide and BSA Free [NBP2-81087]

Immunocytochemistry/ Immunofluorescence: Integrin beta 7 Antibody (FIB27) - Chimeric - Azide and BSA Free [NBP2-81087]

Immunocytochemistry/Immunofluorescence: Integrin beta 7 Antibody (FIB27) [NBP2-81087] - Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with Integrin beta 7 Antibody (FIB27) [NBP2-81087]. Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB27 [NBP2-81087] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81087, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

Applications for Integrin beta 7 Antibody (FIB27) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. FIB27 can be used for FC, IP and can block TKI cell aggregation and beta-7 intergrin-mediated cell adhesion.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Integrin beta 7

Integrin beta7 is a 130 kD glycoprotein, also known as integin betap. It is a member of the Ig superfamily. In association with integrin alpha4 or alphaE chain, beta7 forms alpha4/ beta7 or alphaE/ beta7 heterodimer. alpha4/ beta7 (CD49d/ beta7, LPAM-1) is expressed on majority of peripheral lymphocytes, small subsets of thymocytes and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion, some hematopoietic progenitor cells migration. alphaE/ beta7 (CD103/ beta7, alphaIEL/ beta7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103/ beta7 complex is thought to play a role in lymphocyte retention via interaction with its lignd E-Cadherin. The Fib504 antibody has been reported to react with mouse and human beta7 integrin and to block beta7 integrin-mediated cell adhesion in in vitro and in vivo studies.

Alternate Names

ITGB7

Gene Symbol

ITGB7

Additional Integrin beta 7 Products

Product Documents for Integrin beta 7 Antibody (FIB27) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin beta 7 Antibody (FIB27) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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