Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Intersectin 1 Antibodies »

Product Summary for Intersectin 1 Antibody

Immunogen

The immunogen for this product maps to a region between residue 850 and 900 of human Intersectin 1 using the numbering given in entry NP_003015.2 (GeneID 6453).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Intersectin 1 Antibody

Western Blot: Intersectin 1 Antibody [NBP1-71832]

Western Blot: Intersectin 1 Antibody [NBP1-71832]

Western Blot: Intersectin 1 Antibody [NBP1-71832] - Detection of human and mouse Intersectin-1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Intersectin-1 antibody NBP1-71832 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Previous
Immunoprecipitation: Intersectin 1 Antibody [NBP1-71832]

Immunoprecipitation: Intersectin 1 Antibody [NBP1-71832]

Immunoprecipitation: Intersectin 1 Antibody [NBP1-71832] - Detection of human Intersectin-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Intersectin-1 antibody NBP1-71832 (lot NBP1-71832-2) used for IP at 6 ug per reaction. Intersectin-1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-71832-1). For blotting immunoprecipitated Intersectin-1, NBP1-71832 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for Intersectin 1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Intersectin 1

Intersectin 1 is an adapter protein that may provide an indirect link between the endocytic membrane traffic and the actin assembly machinery. It may regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Intersectin 1 is overexpressed in brain from Down syndrome foetuses suggesting a gene dosage-dependent contribution to the abnormalities of Down syndrome. Intersectin 1 interacts with dynamin, CDC42, SNAP25 and SNAP23. It clusters several dynamin in a manner that is regulated by alternative splicing. Intersectin 1 also binds clathrin-associated proteins and other components of the endocytic machinery, such as SPIN90, EPS15, EPN1, EPN2 and STN2. Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling.

Alternate Names

11, intersectin 1 (SH3 domain protein), intersectin short variant 12, intersectin-1, ITSN, SH3 domain protein-1A, SH3 domain-containing protein 1A, Src homology 3 domain-containing protein

Entrez Gene IDs

6453 (Human)

Gene Symbol

ITSN1

UniProt

Q15811 (Human)

Additional Intersectin 1 Products

Product Documents for Intersectin 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Intersectin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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