Intersectin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87806

Novus Biologicals, part of Bio-Techne
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NBP1-87806
NBP1-87806-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Intersectin 1 Antibodies »

Product Summary for Intersectin 1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWW

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Intersectin 1 Antibody

Western Blot: Intersectin 1 Antibody [NBP1-87806]

Western Blot: Intersectin 1 Antibody [NBP1-87806]

Western Blot: Intersectin 1 Antibody [NBP1-87806] - Analysis in human cell line NTERA-2.
Immunohistochemistry-Paraffin: Intersectin 1 Antibody [NBP1-87806]

Immunohistochemistry-Paraffin: Intersectin 1 Antibody [NBP1-87806]

Immunohistochemistry-Paraffin: Intersectin 1 Antibody [NBP1-87806] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Applications for Intersectin 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Intersectin 1

Intersectin 1 is an adapter protein that may provide an indirect link between the endocytic membrane traffic and the actin assembly machinery. It may regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Intersectin 1 is overexpressed in brain from Down syndrome foetuses suggesting a gene dosage-dependent contribution to the abnormalities of Down syndrome. Intersectin 1 interacts with dynamin, CDC42, SNAP25 and SNAP23. It clusters several dynamin in a manner that is regulated by alternative splicing. Intersectin 1 also binds clathrin-associated proteins and other components of the endocytic machinery, such as SPIN90, EPS15, EPN1, EPN2 and STN2. Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling.

Alternate Names

11, intersectin 1 (SH3 domain protein), intersectin short variant 12, intersectin-1, ITSN, SH3 domain protein-1A, SH3 domain-containing protein 1A, Src homology 3 domain-containing protein

Gene Symbol

ITSN1

Additional Intersectin 1 Products

Product Documents for Intersectin 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Intersectin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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