Intra Acrosomal Protein Antibody (Ds-2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-436
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Canine
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # Ds-2
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for Intra Acrosomal Protein Antibody (Ds-2) - BSA Free
Immunogen
Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
Specificity
The antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications for Intra Acrosomal Protein Antibody (Ds-2) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1:1000
Application Notes
Immunocytochemistry: Membrane permeabilization (acetone) is essential.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Intra Acrosomal Protein
Alternate Names
acrosomal protein SP-10, acrosomal vesicle protein 1D11S4365, SP-10, SPACA2, sperm protein 10
Gene Symbol
ACRV1
Additional Intra Acrosomal Protein Products
Product Documents for Intra Acrosomal Protein Antibody (Ds-2) - BSA Free
Product Specific Notices for Intra Acrosomal Protein Antibody (Ds-2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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