IP3R1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-5908

Novus Biologicals, part of Bio-Techne
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NB120-5908
NB120-5908-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all IP3R1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues N(1829) K K K D D E V D R D A P S R K K A K E(1848) of human IP3 Receptor Type I.

Reactivity Notes

Human reactivity reported by a customer review. Mouse reactivity reported in scientific literature (PMID: 19382908). Rat reactivity reported in scientific literature (PMID: 12767046).

Localization

Endoplasmic reticulum - Integral membrane protein.

Specificity

IP3R1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IP3R1 Antibody

Western Blot: IP3R1 Antibody [NB120-5908]

Western Blot: IP3R1 Antibody [NB120-5908]

Western Blot: IP3R1 Antibody [NB120-5908] - G) Immunoblots of control (C) and proband (P1, P2, P3) cell lysates demonstrate equivalent levels of SERCA2b and IP3R1 Ca2+ channels using rabbit anti-SERCA2 (Novus Biologicals; NBP1-59203) and rabbit anti-IP3R1 (Novus Biologicals; NB120-5908). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27441836) licensed under a CC-BY license.

Applications for IP3R1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
WB: Detects an approx. 240 - 260 kDa protein representing IP3R-I from rat and mouse brain extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB120-5908 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole serum diluted in PBS

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IP3R1

Inositol 1,4,5-trisphosphate (IP3) is a second messenger for many growth factors, hormones, and neuro-transmitters. Upon binding to the IP3 receptor (IP3R), IP3 triggers the release of intracellular, luminal calcium to the cytosol. Functional IP3R is a homo- or heterotetramer of ~240 kDa glycoprotein subunits, that is structurally reminiscent of members of the voltage-gated ion channel superfamily. IP3R protein is structurally and functionally related to one other important intracellular calcium-release channel, the ryanodine receptor. The similarity between the two receptors continues at the physiological level owing to a physical association each receptor can have with the immunophilin protein, FKBP12. Cytosolic calcium levels appear to be regulated by a feedback loop that starts with calcium activation of protein kinase C (PKC) and calcineurin, a protein phosphatase. Phosphorylation of the IP3R by PKC causes an increase in IP3-mediated calcium release. Concomitantly, the phosphatase activity of calcineurin is stimulated upon its association with the FKBP12-IP3R complex. Calcium release is reduced when the PKC target site on the IP3R is dephosphorylated by calcineurin resulting in calcium oscillations. Mammalian IP3R subunits are the product of three distinct genes that are widely expressed and differentially regulated. Type-I IP3R has been detected in heart, liver, kidney, ovary, and Purkinje neurons of the cerebellum. IP3R-II protein is found predominantly in the brain. Isoform III expression has been shown to occur in pancreatic islets, kidney and the gastrointestinal tract.

Long Name

Inositol 1,4,5-trisphosphate receptor type 1

Alternate Names

InsP3R1, IP3R 1, ITPR1

Entrez Gene IDs

3708 (Human); 16438 (Mouse); 25262 (Rat)

Gene Symbol

ITPR1

UniProt

Q14643 (Human)

Additional IP3R1 Products

Product Documents for IP3R1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IP3R1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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