IQGAP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06529
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human motif containing GTPase activating protein 1 using the numbering given in entry NP_003861.1 (GeneID 8826).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IQGAP1 Antibody
![Western Blot: IQGAP1 Antibody [NBP1-06529]](https://resources.bio-techne.com/images/products/IQGAP1-Antibody-Western-Blot-NBP1-06529-img0011.jpg "Western Blot: IQGAP1 Antibody [NBP1-06529]")
Western Blot: IQGAP1 Antibody [NBP1-06529]
Western Blot: IQGAP1 Antibody [NBP1-06529] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IQGAP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-06529]](https://resources.bio-techne.com/images/products/IQGAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-06529-img0009.jpg "Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-06529]")
Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-06529]
Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-06529] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-IQGAP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB![Immunoprecipitation: IQGAP1 Antibody [NBP1-06529]](https://resources.bio-techne.com/images/products/IQGAP1-Antibody-Immunoprecipitation-NBP1-06529-img0010.jpg "Immunoprecipitation: IQGAP1 Antibody [NBP1-06529]")
Immunoprecipitation: IQGAP1 Antibody [NBP1-06529]
Immunoprecipitation: IQGAP1 Antibody [NBP1-06529] - Detection of human IQGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IQGAP1 antibody NBP1-06529 (lot NBP1-06529-2) used for IP at 3 ug per reaction. IQGAP1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06529-1). For blotting immunoprecipitated IQGAP1, NBP1-06529 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for IQGAP1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NBP1-06529 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IQGAP1
Long Name
IQ Motif Containing GTPase Activating Protein 1
Alternate Names
p195, SAR1
Entrez Gene IDs
8826 (Human)
Gene Symbol
IQGAP1
UniProt
Additional IQGAP1 Products
Product Documents for IQGAP1 Antibody
Product Specific Notices for IQGAP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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