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IRF1 Antibody (2E4)

Novus Biologicals, part of Bio-Techne | Catalog # H00003659-M01

Novus Biologicals, part of Bio-Techne
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H00003659-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

IRF1 (NP_002189, 216 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PMPSTSEATTDEDEEGKLPEDIMKLLEQSEWQPTNVDGKGYLLNEPGVQPTSVYGDFSCKEEPEIDSPGGDIGLSLQRVFTDLKNMDATWLDSLLTPVRLPSIQAIPCAP

Localization

Nuclear

Specificity

IRF1 - interferon regulatory factor 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for IRF1 Antibody (2E4)

Western Blot: IRF1 Antibody (2E4) [H00003659-M01]

Western Blot: IRF1 Antibody (2E4) [H00003659-M01]

Western Blot: IRF1 Antibody (2E4) [H00003659-M01] - IRF1 monoclonal antibody (M01), clone 2E4 Analysis of IRF1 expression in COLO 320 HSR.
Sandwich ELISA: IRF1 Antibody (2E4) [H00003659-M01] - Detection limit for recombinant GST tagged IRF1 is approximately 0.1ng/ml as a capture antibody.

Applications for IRF1 Antibody (2E4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IRF1

IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.

Long Name

Interferon Regulatory Factor 1

Alternate Names

MAR

Entrez Gene IDs

3659 (Human)

Gene Symbol

IRF1

OMIM

137215 (Human)

Additional IRF1 Products

Product Documents for IRF1 Antibody (2E4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IRF1 Antibody (2E4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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