Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all IRF1 Antibodies »

Product Summary for IRF1 Antibody

Immunogen

The immunogen for this product maps to a region between residue 300 and 329 of mouse Interferon Regulatory Factor 1 using the numbering given in entry NP_032416.1 (GeneID 16362).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRF1 Antibody

Western Blot: IRF1 Antibody [NBP1-78763]

Western Blot: IRF1 Antibody [NBP1-78763]

Western Blot: IRF1 Antibody [NBP1-78763] - Whole cell lysate from NIH3T3 (M; 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-IRF1 antibody Aused for WB at 0.2 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78761 and NBP1-78762, which recognize disparate epitopes.

Applications for IRF1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IRF1

IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.

Long Name

Interferon Regulatory Factor 1

Alternate Names

MAR

Gene Symbol

IRF1

UniProt

NP_032416.1 (Mouse)

Additional IRF1 Products

Product Documents for IRF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IRF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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