Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockout Validated, Single Cell Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F10

Format

BSA Free

Concentration

1.0 mg/ml

View all IRF3 Antibodies »

Product Specifications

Immunogen

Recombinant human IRF3 (108-166aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IRF3 Antibody (3F10) - BSA Free

Knockout Validated: IRF3 Antibody (3F10) - BSA Free [NBP1-04308]

Knockout Validated: IRF3 Antibody (3F10) - BSA Free [NBP1-04308]

Knockout Validated: IRF3 Antibody (3F10) [NBP1-04308] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and IRF3 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human IRF3 Monoclonal Antibody (Catalog # NBP1-04308) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for IRF3 at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: IRF3 Antibody (3F10)BSA Free [NBP1-04308]

Western Blot: IRF3 Antibody (3F10)BSA Free [NBP1-04308]

Western Blot: IRF3 Antibody (3F10) - BSA Free [NBP1-04308] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: MCF7 cell lysate. Lane 2: K562 cell lysate. Lane 3: Jurkat cell lysate
Immunocytochemistry/ Immunofluorescence: IRF3 Antibody (3F10) - BSA Free [NBP1-04308]

Immunocytochemistry/ Immunofluorescence: IRF3 Antibody (3F10) - BSA Free [NBP1-04308]

Immunocytochemistry/Immunofluorescence: IRF3 Antibody (3F10) [NBP1-04308] - Human HeLa cells stained with IRF-3 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.
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Applications for IRF3 Antibody (3F10) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:500

Single Cell Western

100 ug/ml

Western Blot

1:1000-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRF3

Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is a member of the IRF family, and is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection.

Long Name

Interferon Regulatory Factor 3

Alternate Names

interferon regulatory factor 3, IRF-3

Gene Symbol

IRF3

UniProt

NP_001562 (Human)

Additional IRF3 Products

Product Documents for IRF3 Antibody (3F10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IRF3 Antibody (3F10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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