IRF3 Antibody (8D3Y4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15877

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15877-100ul
NBP3-15877-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8D3Y4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IRF3 Antibodies »

Product Summary for IRF3 Antibody (8D3Y4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IRF3 (Q14653). DFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRF3 Antibody (8D3Y4)

Western Blot: IRF3 Antibody (8D3Y4) [NBP3-15877]

Western Blot: IRF3 Antibody (8D3Y4) [NBP3-15877]

Western Blot: IRF3 Antibody (8D3Y4) [NBP3-15877] - Western blot analysis of extracts of various cell lines, using IRF3 antibody (NBP3-15877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Knockout Validated: IRF3 Antibody (8D3Y4) [NBP3-15877]

Knockout Validated: IRF3 Antibody (8D3Y4) [NBP3-15877]

Knockout Validated: IRF3 Antibody (8D3Y4) [NBP3-15877] - Western blot analysis of extracts from normal (control) and IRF3 knockout (KO) HeLa cells, using IRF3 antibody (NBP3-15877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for IRF3 Antibody (8D3Y4)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IRF3

IRF3 is a family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. he high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3).

Long Name

Interferon Regulatory Factor 3

Alternate Names

interferon regulatory factor 3, IRF-3

Gene Symbol

IRF3

Additional IRF3 Products

Product Documents for IRF3 Antibody (8D3Y4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IRF3 Antibody (8D3Y4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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