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IRF7 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06987

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the center of human IRF7. The immunogen is located within amino acids 200-230 of IRF7.

Specificity

Several isoforms of IRF7 are known to exist. Human IRF7 has 4 isoforms, including isoform A (503aa, 54kD), isoform B (474aa, 52kD), isoform C (164aa, 18kD) and isoform D (516aa, 56kD). Mouse IRF7 has one isoform (457aa, 51kD) and Rat IRF7 also has one isoform (456aa, 51kD). NBP3-06987 can detect human isoforms and mouse, and possibly can detect rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRF7 Antibody - BSA Free

Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]

Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]

Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution
Immunocytochemistry/ Immunofluorescence: IRF7 Antibody - BSA Free [NBP3-06987]

Immunocytochemistry/ Immunofluorescence: IRF7 Antibody - BSA Free [NBP3-06987]

Immunocytochemistry/Immunofluorescence: IRF7 Antibody [NBP3-06987] - Immunofluorescent analysis of 4% paraformaldehyde-fixed Raw264.7 cells labeling IRF7 with NBP3-06987 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987]

Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987]

Immunohistochemistry: IRF7 Antibody [NBP3-06987] - Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using NBP3-06987 at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degrees C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for IRF7 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2-5 ug/mL

Immunohistochemistry-Paraffin

2-5 ug/mL

Western Blot

0.5-1 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRF7

IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.

Alternate Names

interferon regulatory factor 7, interferon regulatory factor-7H, IRF-7, IRF7A, IRF-7H

Gene Symbol

IRF7

Additional IRF7 Products

Product Documents for IRF7 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IRF7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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