IRF8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83123

Novus Biologicals, part of Bio-Techne
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NBP1-83123
NBP1-83123-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IRF8 Antibodies »

Product Summary for IRF8 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PDFEEVTDRSQLDISEPYKVYRIVPEEEQKCKLGVATAGCVNEVTEMECGRSEIDELIKEPSVDDYMGMIKRSPSPPEACRSQLLPDWWAQQPSTGVPLVTGYTTYDAHHSAFSQMVISFYY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRF8 Antibody

Immunohistochemistry-Paraffin: IRF8 Antibody [NBP1-83123]

Immunohistochemistry-Paraffin: IRF8 Antibody [NBP1-83123]

Immunohistochemistry-Paraffin: IRF8 Antibody [NBP1-83123] - Analysis in human tonsil and skeletal muscle tissues using NBP1-83123 antibody. Corresponding IRF8 RNA-seq data are presented for the same tissues.
Western Blot: IRF8 Antibody [NBP1-83123]

Western Blot: IRF8 Antibody [NBP1-83123]

Western Blot: IRF8 Antibody [NBP1-83123] - Analysis in human cell line THP-1.
Immunocytochemistry/ Immunofluorescence: IRF8 Antibody [NBP1-83123]

Immunocytochemistry/ Immunofluorescence: IRF8 Antibody [NBP1-83123]

Immunocytochemistry/Immunofluorescence: IRF8 Antibody [NBP1-83123] - Staining of human cell line CACO-2 shows localization to nucleoplasm.
View 2 more images

Applications for IRF8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRF8

Interferon regulatory factor 8 (IRF8) / Interferon consensus sequence binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA binding domain in the N terminal region and a divergent C terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN alpha and IFN beta. IRF family proteins also control expression of IFN alpha and IFN beta regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN inducible MHC class I genes (the interferon consensus sequence (ICS)). Unlike IRF3, IRF8 appears to act as a negative regulator of IFN induced genes in most cases, but IRF8 mediates activation of NF kappaB by the toll like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia.

Long Name

Interferon Regulatory Factor 8

Alternate Names

ICSBP1

Gene Symbol

IRF8

Additional IRF8 Products

Product Documents for IRF8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IRF8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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