IRF9 Antibody (PCRP-IRF9-2F8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13835

Novus Biologicals, part of Bio-Techne
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NBP3-13835-100ug
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-IRF9-2F8

Concentration

0.2 mg/ml

View all IRF9 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human IRF9 protein (Uniprot: Q00978)

Localization

Cytoplasm, Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

43.34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14228)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for IRF9 Antibody (PCRP-IRF9-2F8)

Immunocytochemistry/ Immunofluorescence: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835]

Immunocytochemistry/ Immunofluorescence: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835]

Immunocytochemistry/Immunofluorescence: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835] - Immunofluorescent analysis of PFA-fixed HeLa cells. IRF9 Antibody (PCRP-IRF9-2F8) followed by goat anti-mouse IgG-CF488 (green); counterstain (red).
Flow Cytometry: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835]

Flow Cytometry: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835]

Flow Cytometry: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835] - Flow cytometric analysis of PFA-fixed HeLa cells. IRF9 antibody (PCRP-IRF9-2F8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835]

Protein Array: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835]

Protein Array: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IRF9 Mouse Monoclonal Antibody (PCRP-IRF9-2F8).

Applications for IRF9 Antibody (PCRP-IRF9-2F8)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IRF9

Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state

Long Name

Interferon Regulatory Factor 9

Alternate Names

ISGF3, ISGF3G

Gene Symbol

IRF9

UniProt

Q00978

Additional IRF9 Products

Product Documents for IRF9 Antibody (PCRP-IRF9-2F8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IRF9 Antibody (PCRP-IRF9-2F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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