IRP2 Antibody (9D1-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32483
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 9D1-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human IRP2 aa 1-963. (Uniprot: P48200)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IRP2 Antibody (9D1-R)
Western Blot: IRP2 Antibody (9D1-R) [NBP3-32483]
Western Blot: IRP2 Antibody (9D1-R) [NBP3-32483] - Western blot analysis of IRP2 on different lysates with Mouse anti-IRP2 antibody (NBP3-32483) at 1/2,000 dilution.Lane 1: Daudi cell lysate (20 ug/Lane)
Lane 2: A549 cell lysate (20 ug/Lane)
Lane 3: Mouse liver tissue lysate (40 ug/Lane)
Lane 4: Mouse kidney tissue lysate (40 ug/Lane)
Lane 5: Mouse heart tissue lysate (40 ug/Lane)
Lane 6: Rat liver tissue lysate (40 ug/Lane)
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32483) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Applications for IRP2 Antibody (9D1-R)
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IRP2
Alternate Names
ACO3, EC 4.2.1.3, FLJ23381, IRE-BP 2, Iron regulatory protein 2, iron-responsive element binding protein 2, iron-responsive element-binding protein 2, IRP2IRP2AD
Gene Symbol
IREB2
Additional IRP2 Products
Product Documents for IRP2 Antibody (9D1-R)
Product Specific Notices for IRP2 Antibody (9D1-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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