Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all IRS1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-280 of human IRS1(NP_005535) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

131.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IRS1 Antibody (OTI3G10) - Azide and BSA Free

Western Blot: IRS1 Antibody (OTI3G10)Azide and BSA Free [NBP2-71049]

Western Blot: IRS1 Antibody (OTI3G10)Azide and BSA Free [NBP2-71049]

Western Blot: IRS1 Antibody (OTI3G10) - Azide and BSA Free [NBP2-71049] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRS1.
Immunohistochemistry: IRS1 Antibody (OTI3G10) - Azide and BSA Free [NBP2-71049]

Immunohistochemistry: IRS1 Antibody (OTI3G10) - Azide and BSA Free [NBP2-71049]

Immunohistochemistry: IRS1 Antibody (OTI3G10) - Azide and BSA Free [NBP2-71049] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: IRS1 Antibody (OTI3G10) - Azide and BSA Free [NBP2-71049]

Immunohistochemistry: IRS1 Antibody (OTI3G10) - Azide and BSA Free [NBP2-71049]

Immunohistochemistry: IRS1 Antibody (OTI3G10) - Azide and BSA Free [NBP2-71049] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for IRS1 Antibody (OTI3G10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IRS1

Insulin receptor substrates (IRS), the major intracellular substrates of the insulin receptor (IR), are adaptor proteins that transduce signals from the IR to downstream effectors that are important for the biological effect of insulin (1-2). After insulin stimulation, IRS proteins are rapidly phosphorylated on multiple tyrosine residues. Once phosphorylated, IRS proteins bind and activate Grb-2, SHP2 and the PI3-K p85 subunit (2-3). IRS-1 functions as one of the key regulators of IR and the insulin-like growth factor-1 receptor. Disruption of IRS-1 causes growth retardation and insulin resistance associated with hypertension, hypertriglyceridemia, and impaired endothelium-dependent vascular relaxation (4-5)

Long Name

Insulin Receptor Substrate 1

Alternate Names

HIRS-1, insulin receptor substrate 1, IRS-1

Gene Symbol

IRS1

Additional IRS1 Products

Product Documents for IRS1 Antibody (OTI3G10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IRS1 Antibody (OTI3G10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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