Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all IRS1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the center of human IRS-1. The immunogen is located within amino acids 910 - 960 of IRS-1. Amino Acid Squence: QPAPREEETGTEEYMK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRS1 Antibody - BSA Free

Western Blot: IRS1 AntibodyBSA Free [NBP1-77216]

Western Blot: IRS1 AntibodyBSA Free [NBP1-77216]

Western Blot: IRS1 Antibody [NBP1-77216] - PC-3 cell lysate with IRS-1 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: IRS1 Antibody - BSA Free [NBP1-77216]

Immunocytochemistry/ Immunofluorescence: IRS1 Antibody - BSA Free [NBP1-77216]

Immunocytochemistry/Immunofluorescence: IRS1 Antibody [NBP1-77216] - P815 cells at 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: IRS1 Antibody - BSA Free [NBP1-77216]

Immunocytochemistry/ Immunofluorescence: IRS1 Antibody - BSA Free [NBP1-77216]

Immunocytochemistry/Immunofluorescence: IRS1 Antibody [NBP1-77216] - P815 cells with IRS-1 antibody at 2 ug/ml.
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Applications for IRS1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2 ug/ml

Western Blot

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRS1

Following tyrosine phosphorylation, the insulin receptor substrate 1 and 2 (IRS-1 and IRS-2) can form a protein scaffolding for the assembly of a host of Src homology 2 (SH2) domain-containing proteins. IRS-1 tyrosine phosphorylation can occur through the activity of several cytokine and growth factor receptors such as interleukin (IL)-4, IL-9, interferon-gamma, in addition to the insulin and insulin-like growth factor 1 receptors. The scaffolding provided by IRS-1 and IRS-2 is necessary for insulin signal transduction across the cell membrane. IRS-1 tyrosine phosphorylation, and thus formation of the IRS scaffolding is inhibited by tumor necrosis factor (TNF), and this inhibition can itself be blocked by rapamycin, an inhibitor of the mammalian target of rapamycin (TOR). TNF activity could also be blocked by inhibition of the Akt kinase and the PTEN tumor suppressor, suggesting that TNF impairs insulin signaling through IRS-1 by activation of the TOR signaling pathway.

Long Name

Insulin Receptor Substrate 1

Alternate Names

HIRS-1, insulin receptor substrate 1, IRS-1

Gene Symbol

IRS1

UniProt

P35568

Additional IRS1 Products

Product Documents for IRS1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IRS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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