IRS4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29452

Novus Biologicals, part of Bio-Techne
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NBP3-29452
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IRS4 Antibodies »

Product Specifications

Immunogen

Between 875 and 925

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRS4 Antibody

IRS4 Antibody

Immunoprecipitation:IRS4 AntibodyNBP3-29452] -

Immunoprecipitation:IRS4 AntibodyNBP3-29452] -Detection of human IRS4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IRS4 antibody NBP3-29452 (lot NBP3-29452-1) used for IP at 6 µg per reaction. IRS4 was also immunoprecipitated by rabbit anti-IRS4.. For blotting immunoprecipitated IRS4, NBP3-29452 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for IRS4 Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IRS4

IRS4 is a new member of the IRS family, IRS-4, is a 160-kDa tyrosine kinase which phosphorylates cellular substrates when it is activated by the binding of insulin (1). IRS-4 was originally discovered in human embryonic kidney 293 cells. Subcellular fractionation revealed that about 50% of IRS-4 was located in cellular membranes, and immunofluorescence indicated that IRS-4 was concentrated at the plasma membrane. Immunoelectron microscopy conclusively established that a large portion of the IRS-4 was located at the cytoplasmic surface of the plasma membrane in both the unstimulated and insulin-treated states. IRS-4 was found to be associated with two src homology 2 (SH2) domain-containing proteins, phosphatidylinositol 3-kinase and Grb2, the adaptor to the guanine nucleotide exchange factor for Ras (2).

Alternate Names

insulin receptor substrate 4,160 kDa phosphotyrosine protein, IRS-4pp160, Phosphoprotein of 160 kDa, PY160

Gene Symbol

IRS4

Additional IRS4 Products

Product Documents for IRS4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IRS4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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