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ISCU Antibody (3B8-1C4)

Novus Biologicals, part of Bio-Techne | Catalog # H00023479-M01

Novus Biologicals, part of Bio-Techne
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H00023479-M01

Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B8-1C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NIFUN (AAH11906, 26 a.a. ~ 167 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAEKK

Reactivity Notes

Human. Other species not tested.

Specificity

NIFUN - NifU-like N-terminal domain containing

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ISCU Antibody (3B8-1C4)

Western Blot: ISCU Antibody (3B8-1C4) [H00023479-M01]

Western Blot: ISCU Antibody (3B8-1C4) [H00023479-M01]

Western Blot: ISCU Antibody (3B8-1C4) [H00023479-M01] - NIFUN monoclonal antibody (M01), clone 3B8-1C4 Analysis of NIFUN expression in HL-60.
Immunocytochemistry/ Immunofluorescence: ISCU Antibody (3B8-1C4) [H00023479-M01]

Immunocytochemistry/ Immunofluorescence: ISCU Antibody (3B8-1C4) [H00023479-M01]

Immunocytochemistry/Immunofluorescence: ISCU Antibody (3B8-1C4) [H00023479-M01] - Analysis of monoclonal antibody to NIFUN on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: ISCU Antibody (3B8-1C4) [H00023479-M01] - Detection limit for recombinant GST tagged NIFUN is approximately 0.03ng/ml as a capture antibody.

Applications for ISCU Antibody (3B8-1C4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ISCU

Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria.

Alternate Names

2310020H20Rik, HML, hnifU, iron-sulfur cluster assembly enzyme ISCU, mitochondrial, iron-sulfur cluster scaffold homolog (E. coli), IscU, IscU iron-sulfur cluster scaffold homolog, IscU iron-sulfur cluster scaffold homolog (E. coli), ISU2, MGC74517, NIFU, NifU-like N-terminal domain containing, NifU-like N-terminal domain-containing protein, NifU-like protein, NIFUN

Entrez Gene IDs

23479 (Human)

Gene Symbol

ISCU

Additional ISCU Products

Product Documents for ISCU Antibody (3B8-1C4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ISCU Antibody (3B8-1C4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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