ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33446
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 6V3Y5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human ISG15 Activating Enzyme/UBE1L (NP_003326.2).
Sequence:
MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNR
Sequence:
MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNR
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5)
Western Blot: ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5) [NBP3-33446] -
Western Blot: ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5) [NBP3-33446] - Western blot analysis of lysates from HEL cells, using ISG15 Activating Enzyme/UBE1L Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Applications for ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ISG15 Activating Enzyme/UBE1L
Alternate Names
D8, UBA1B, UBA7, UBE2
Gene Symbol
UBA7
Additional ISG15 Activating Enzyme/UBE1L Products
Product Documents for ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5)
Product Specific Notices for ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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