ISG15 Activating Enzyme/UBE1L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32579

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ISG15 Activating Enzyme/UBE1L Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ISG15 Activating Enzyme/UBE1L. The exact sequence is proprietary.

Reactivity Notes

Cat (84%), Bovine (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ISG15 Activating Enzyme/UBE1L Antibody

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [NBP1-32579]

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [NBP1-32579]

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [NBP1-32579] - (30 ug whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000
Immunohistochemistry-Paraffin: ISG15 Activating Enzyme/UBE1L Antibody [NBP1-32579]

Immunohistochemistry-Paraffin: ISG15 Activating Enzyme/UBE1L Antibody [NBP1-32579]

Immunohistochemistry-Paraffin: ISG15 Activating Enzyme/UBE1L Antibody [NBP1-32579] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
ISG15 Activating Enzyme/UBE1L Antibody

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [NBP1-32579] -

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [NBP1-32579] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UBE1L antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for ISG15 Activating Enzyme/UBE1L Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ISG15 Activating Enzyme/UBE1L

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein.

Alternate Names

D8, UBA1B, UBA7, UBE2

Gene Symbol

UBA7

Additional ISG15 Activating Enzyme/UBE1L Products

Product Documents for ISG15 Activating Enzyme/UBE1L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ISG15 Activating Enzyme/UBE1L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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